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anti cd16 cd32  (Bio X Cell)


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    Structured Review

    Bio X Cell anti cd16 cd32
    Anti Cd16 Cd32, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 367 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+cd16+cd32/InVivoMAb+anti-mouse+CD16+CD32/pm41912083-75-20-25
    Average 96 stars, based on 367 article reviews
    anti cd16 cd32 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: Purinergic adipocyte-macrophage crosstalk promotes degeneration of thermogenic brown adipose tissue
    Article Snippet: The pellet was re-suspended in FACS buffer containing 0.1% bovine serum albumin (Sigma) and centrifuged at 1600 rpm for 5 min. To lyse erythrocytes, the cell pellets were incubated with lysis buffer (155 mM NH 4 Cl, 10 mM KHCO 3 , 0.1 mM EDTA, pH 7.2) for 3 min at room temperature.

    Article Title: Purinergic adipocyte-macrophage crosstalk promotes degeneration of thermogenic brown adipose tissue.
    Article Snippet: For mRNA sequencing (RNAseq), total RNA was sent to Novogene (UK) for quality check, library construction and transcriptome sequencing of RNA samples on a NovaSeq 6000 PE150 platform.

    Article Title: An interleukin-27-centered cytokine circuit regulates macrophage and T cell interactions in autoimmune diabetes
    Article Snippet: Anti-mouse CD16/CD32 , BioXCell , Clone 2.4G2, Catalog #BE0307; RRID: AB_2736987.

    Incubation:

    Article Title: Temporal single-cell analysis reveals age-associated delay in immune resolution after respiratory viral infection
    Article Snippet: .. Following the Fc receptor blockade with anti-mouse CD16/CD32 (Clone: 2.4G2, Bio X Cell), cells were incubated in MACS-buffer-based solutions with antibodies targeting surface markers on ice for 30min. ..

    Article Title: TCR signal strength determines T reg instability and discrimination of self versus non-self antigens
    Article Snippet: .. For surface staining, cells were first incubated with anti-mouse CD16/CD32 (Fc block; BioXCell) in FACS buffer on ice for 10 min in the dark. .. Subsequently, they were stained for 30 min at 4°C with a mixture of fluorochrome-conjugated antibodies and Fixable Viability Dye eFluor 506 (eBioscience).

    Magnetic Cell Separation:

    Article Title: Temporal single-cell analysis reveals age-associated delay in immune resolution after respiratory viral infection
    Article Snippet: .. Following the Fc receptor blockade with anti-mouse CD16/CD32 (Clone: 2.4G2, Bio X Cell), cells were incubated in MACS-buffer-based solutions with antibodies targeting surface markers on ice for 30min. ..

    Staining:

    Article Title: Orf virus-based vectors induce potent germinal center B cell, Tfh cell, and CD8 + T cell responses.
    Article Snippet: .. 114 Staining was performed in FACS buffer (PBS supplemented with 5% FCS) containing rat IgG (Sigma, I4131) and anti-mouse CD16/CD32 (2.4G2; BioXcell, BE0307) to block unspecific antibody binding. .. Additionally, we added BD Horizon brilliant stain buffer (BD Biosciences, 566385) whenever more than one brilliant violet dye was used in the staining mix.

    Article Title: TCR signal strength determines T reg instability and discrimination of self versus non-self antigens
    Article Snippet: .. For surface staining, cells were first incubated with anti-mouse CD16/CD32 (Fc block; BioXCell) in FACS buffer on ice for 10 min in the dark. .. Subsequently, they were stained for 30 min at 4°C with a mixture of fluorochrome-conjugated antibodies and Fixable Viability Dye eFluor 506 (eBioscience).

    FACS:

    Article Title: Orf virus-based vectors induce potent germinal center B cell, Tfh cell, and CD8 + T cell responses.
    Article Snippet: .. 114 Staining was performed in FACS buffer (PBS supplemented with 5% FCS) containing rat IgG (Sigma, I4131) and anti-mouse CD16/CD32 (2.4G2; BioXcell, BE0307) to block unspecific antibody binding. .. Additionally, we added BD Horizon brilliant stain buffer (BD Biosciences, 566385) whenever more than one brilliant violet dye was used in the staining mix.

    Article Title: TCR signal strength determines T reg instability and discrimination of self versus non-self antigens
    Article Snippet: .. For surface staining, cells were first incubated with anti-mouse CD16/CD32 (Fc block; BioXCell) in FACS buffer on ice for 10 min in the dark. .. Subsequently, they were stained for 30 min at 4°C with a mixture of fluorochrome-conjugated antibodies and Fixable Viability Dye eFluor 506 (eBioscience).

    Article Title: Chronic Infection Perturbs the Affinity Hierarchy of Antiviral B Cells
    Article Snippet: Prior to staining, spleen and lymph nodes suspensions were incubated for 10 min at room temperature (RT) with DNAse I (50 μg/ml; Roche, 10104159001) in FACS buffer. .. Stains were performed in FACS buffer supplemented with polyclonal rat IgG and anti-mouse CD16/CD32 (2.42; BioXcell, BE0307) to block nonspecific antibody binding. .. When more than one brilliant violet dye was in the staining mix, a BD HorizonTM brilliant stain buffer (BD Biosciences, 566385) was added to the staining mix.

    Blocking Assay:

    Article Title: Orf virus-based vectors induce potent germinal center B cell, Tfh cell, and CD8 + T cell responses.
    Article Snippet: .. 114 Staining was performed in FACS buffer (PBS supplemented with 5% FCS) containing rat IgG (Sigma, I4131) and anti-mouse CD16/CD32 (2.4G2; BioXcell, BE0307) to block unspecific antibody binding. .. Additionally, we added BD Horizon brilliant stain buffer (BD Biosciences, 566385) whenever more than one brilliant violet dye was used in the staining mix.

    Article Title: TCR signal strength determines T reg instability and discrimination of self versus non-self antigens
    Article Snippet: .. For surface staining, cells were first incubated with anti-mouse CD16/CD32 (Fc block; BioXCell) in FACS buffer on ice for 10 min in the dark. .. Subsequently, they were stained for 30 min at 4°C with a mixture of fluorochrome-conjugated antibodies and Fixable Viability Dye eFluor 506 (eBioscience).

    Article Title: Chronic Infection Perturbs the Affinity Hierarchy of Antiviral B Cells
    Article Snippet: Prior to staining, spleen and lymph nodes suspensions were incubated for 10 min at room temperature (RT) with DNAse I (50 μg/ml; Roche, 10104159001) in FACS buffer. .. Stains were performed in FACS buffer supplemented with polyclonal rat IgG and anti-mouse CD16/CD32 (2.42; BioXcell, BE0307) to block nonspecific antibody binding. .. When more than one brilliant violet dye was in the staining mix, a BD HorizonTM brilliant stain buffer (BD Biosciences, 566385) was added to the staining mix.

    Binding Assay:

    Article Title: Orf virus-based vectors induce potent germinal center B cell, Tfh cell, and CD8 + T cell responses.
    Article Snippet: .. 114 Staining was performed in FACS buffer (PBS supplemented with 5% FCS) containing rat IgG (Sigma, I4131) and anti-mouse CD16/CD32 (2.4G2; BioXcell, BE0307) to block unspecific antibody binding. .. Additionally, we added BD Horizon brilliant stain buffer (BD Biosciences, 566385) whenever more than one brilliant violet dye was used in the staining mix.

    Article Title: Chronic Infection Perturbs the Affinity Hierarchy of Antiviral B Cells
    Article Snippet: Prior to staining, spleen and lymph nodes suspensions were incubated for 10 min at room temperature (RT) with DNAse I (50 μg/ml; Roche, 10104159001) in FACS buffer. .. Stains were performed in FACS buffer supplemented with polyclonal rat IgG and anti-mouse CD16/CD32 (2.42; BioXcell, BE0307) to block nonspecific antibody binding. .. When more than one brilliant violet dye was in the staining mix, a BD HorizonTM brilliant stain buffer (BD Biosciences, 566385) was added to the staining mix.



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